ASUS introduced its first socket AM4 motherboard bearing the durable TUF branding, the TUF B350M-Plus Gaming. Built in the micro-ATX form-factor, the board draws power from a combination of 24-pin ATX and 8-pin EPS power connectors, conditioning it for the AM4 SoC using a 6-phase VRM. The components that make up the VRM are of a very high grade, enough to warrant TUF branding. The AM4 socket is wired to four DDR4 DIMM slots, supporting up to 64 GB of dual-channel DDR4 memory; and the board's single and reinforced PCI-Express 3.0 x16 slot. The second x16 slot is electrical x4, and wired to the AMD B350 chipset.

Storage connectivity includes one 32 Gb/s M.2 slot, and six SATA 6 Gb/s ports, two of which are directly wired to the SoC. Display connectivity includes one each of HDMI, D-Sub, and DVI. USB connectivity includes two 10 Gb/s USB 3.1 ports (both type-A), eight USB 3.0 ports (six on the rear panel, two by headers). Networking is care of a single 1 GbE interface, driven by a Realtek RTL8111H controller.